Dataset Information

The HOPE fixation technique--a promising alternative to common prostate cancer biobanking approaches.

ABSTRACT:

Background

The availability of well-annotated prostate tissue samples through biobanks is key for research. Whereas fresh-frozen tissue is well suited for a broad spectrum of molecular analyses, its storage and handling is complex and cost-intensive. Formalin-fixed paraffin-embedded specimens (FFPE) are easy to handle and economic to store, but their applicability for molecular methods is restricted. The recently introduced Hepes-glutamic acid-buffer mediated Organic solvent Protection Effect (HOPE) is a promising alternative, which might have the potential to unite the benefits of FFPE and fresh-frozen specimen. Aim of the study was to compare HOPE-fixed, FFPE and fresh-frozen bio-specimens for their accessibility for diagnostic and research purposes.

Methods

10 prostate cancer samples were each preserved with HOPE, formalin, and liquid nitrogen and studied with in-situ and molecular methods. Samples were H&E stained, and assessed by immunohistochemistry (i.e. PSA, GOLPH2, p63) and FISH (i.e. ERG rearrangement). We assessed DNA integrity by PCR, using control genes ranging from 100 to 600 bp amplicon size. RNA integrity was assessed through qRT-PCR on three housekeeping genes (TBP, GAPDH, ?-actin). Protein expression was analysed by performing western blot analysis using GOLPH2 and PSA antibodies.

Results

Of the HOPE samples, morphologic quality of H&E sections, immunohistochemical staining, and the FISH assay was at least equal to FFPE tissue, and significantly better than the fresh-frozen specimens. DNA, RNA, and protein analysis of HOPE samples provided similar results as compared to fresh-frozen specimens. As expected, FFPE-samples were inferior for most of the molecular analyses.

Conclusions

This is the first study, comparatively assessing the suitability of these fixation methods for diagnostic and research utilization. Overall, HOPE-fixed bio-specimens combine the benefits of FFPE- and fresh-frozen samples. Results of this study have the potential to expand on contemporary prostate tissue biobanking approaches and can serve as a model for other organs and tumors.

SUBMITTER: Braun M

PROVIDER: S-EPMC3248383 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Publications

The HOPE fixation technique--a promising alternative to common prostate cancer biobanking approaches.

Braun Martin M Menon Roopika R Nikolov Pavel P Kirsten Robert R Petersen Karen K Schilling David D Schott Christina C Gündisch Sibylle S Fend Falko F Becker Karl-Friedrich KF Perner Sven S

BMC cancer 20111207

<h4>Background</h4>The availability of well-annotated prostate tissue samples through biobanks is key for research. Whereas fresh-frozen tissue is well suited for a broad spectrum of molecular analyses, its storage and handling is complex and cost-intensive. Formalin-fixed paraffin-embedded specimens (FFPE) are easy to handle and economic to store, but their applicability for molecular methods is restricted. The recently introduced Hepes-glutamic acid-buffer mediated Organic solvent Protection E ...[more]

PMID: 22151117

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Dataset Information

The HOPE fixation technique--a promising alternative to common prostate cancer biobanking approaches.

Background

Methods

Results

Conclusions

Publications

The HOPE fixation technique--a promising alternative to common prostate cancer biobanking approaches.

Similar Datasets

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