Decoding cell lineage from acquired mutations using arbitrary deep sequencing.
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ABSTRACT:
SUBMITTER: Carlson CA
PROVIDER: S-EPMC3248619 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Publications
Decoding cell lineage from acquired mutations using arbitrary deep sequencing.
Carlson Cheryl A CA Kas Arnold A Kirkwood Robert R Hays Laura E LE Preston Bradley D BD Salipante Stephen J SJ Horwitz Marshall S MS
Nature methods 20111127 1
Because mutations are inevitable, the genome of each cell in a multicellular organism becomes unique and therefore encodes a record of its ancestry. Here we coupled arbitrary single primer PCR with next-generation DNA sequencing to catalog mutations and deconvolve the phylogeny of cultured mouse cells. This study helps pave the way toward construction of retrospective cell-fate maps based on mutations accumulating in genomes of somatic cells. ...[more]
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