Ontology highlight
ABSTRACT:
SUBMITTER: Chu C
PROVIDER: S-EPMC3249421 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Chu Ci C Qu Kun K Zhong Franklin L FL Artandi Steven E SE Chang Howard Y HY
Molecular cell 20110929 4
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and DNA sequences, which are enumerated by deep sequencing. ChIRP-seq of three lncRNAs reveal that RNA occupancy sites in the genome are focal, sequence-specific, and numerous. Drosophila roX2 RNA occupies male X-li ...[more]