Ontology highlight
ABSTRACT:
SUBMITTER: Nagai T
PROVIDER: S-EPMC3251075 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Nagai Takuya T Unno Hideaki H Janczak Matthew Walter MW Yoshimura Tohru T Poulter C Dale CD Hemmi Hisashi H
Proceedings of the National Academy of Sciences of the United States of America 20111207 51
Evidence for an unusual catalysis of protonation/deprotonation by a reduced flavin mononucleotide cofactor is presented for type-2 isopentenyl diphosphate isomerase (IDI-2), which catalyzes isomerization of the two fundamental building blocks of isoprenoid biosynthesis, isopentenyl diphosphate and dimethylallyl diphosphate. The covalent adducts formed between irreversible mechanism-based inhibitors, 3-methylene-4-penten-1-yl diphosphate or 3-oxiranyl-3-buten-1-yl diphosphate, and the flavin cofa ...[more]