Increased serum soluble IL-15R? levels in T-cell large granular lymphocyte leukemia.

ABSTRACT: Large granular lymphocyte (LGL) leukemia is a clonal lymphoproliferative disease of mature T and natural killer cells. The etiology of LGL leukemia is unknown. IL-15 is an inflammatory cytokine that stimulates T and natural killer cells and is critical for their survival and proliferation. IL-15 signals through a heterotrimeric receptor that is composed of a private receptor, IL-15R? and IL-2/IL-15R? and ?(c) shared with IL-2. Using a newly developed assay, we demonstrated increased levels of soluble IL-15R? in the serum of patients with T-LGL leukemia. Furthermore, IL-15R? mRNA levels were also up-regulated in the PBMCs of these patients. FACS analysis indicated that IL-15R? was expressed both on monocytes as well as on some CD8+ leukemic cells of the patients. Interestingly, the mRNA levels of IFN-?, a known inducer of IL-15R?, were also up-regulated in patients' PBMCs. Moreover, PBMCs of some T-LGL patients proliferated at higher levels in response to exogenously added IL-15 compared with those of normal donors. In summary, our study demonstrated increased expression of IL-15R? in T-LGL leukemia. It is conceivable that higher IL-15R? expression may lower IL-15 response threshold in vivo and, therefore, may contribute to the pathogenesis of the disease.

SUBMITTER: Chen J 

PROVIDER: S-EPMC3251226 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature