Escherichia coli SlyD, more than a Ni(II) reservoir.
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ABSTRACT:
SUBMITTER: Kaluarachchi H
PROVIDER: S-EPMC3253374 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Publications
Escherichia coli SlyD, more than a Ni(II) reservoir.
Kaluarachchi Harini H Zhang Jei Wei JW Zamble Deborah B DB
Biochemistry 20111118 50
SlyD interacts with HypB and contributes to nickel insertion during [NiFe]-hydrogenase biogenesis. Herein, we provide evidence of SlyD acting as a nickel storage determinant in Escherichia coli and show that this Ni(II) can be mobilized to HypB in vitro even under competitive conditions. Furthermore, SlyD enhances the GTPase activity of HypB, and acceleration of release of Ni(II) from HypB is more pronounced when HypB is GDP-bound. The data support a model in which a HypB-SlyD complex establishe ...[more]
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