Hydride transfer versus hydrogen radical transfer in thymidylate synthase.
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ABSTRACT:
SUBMITTER: Hong B
PROVIDER: S-EPMC3253763 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Publications
Hydride transfer versus hydrogen radical transfer in thymidylate synthase.
Hong Baoyu B Haddad Majd M Maley Frank F Jensen Jan H JH Kohen Amnon A
Journal of the American Chemical Society 20060501 17
The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer. ...[more]
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