Ontology highlight
ABSTRACT:
SUBMITTER: Puig-Saus C
PROVIDER: S-EPMC3255593 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Puig-Saus Cristina C Gros Alena A Alemany Ramon R Cascalló Manel M
Molecular therapy : the journal of the American Society of Gene Therapy 20110823 1
Tumor-associated stromal cells constitute a major hurdle in the antitumor efficacy with oncolytic adenoviruses. To overcome this biological barrier, an in vitro bioselection of a mutagenized AdwtRGD stock in human cancer-associated fibroblasts (CAFs) was performed. Several rounds of harvest at early cytopathic effect (CPE) followed by plaque isolation led us to identify one mutant with large plaque phenotype, enhanced release in CAFs and enhanced cytotoxicity in CAF and several tumor cell lines. ...[more]