Expression vectors for Acinetobacter baylyi ADP1.
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ABSTRACT:
SUBMITTER: Murin CD
PROVIDER: S-EPMC3255645 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Publications
Expression vectors for Acinetobacter baylyi ADP1.
Murin Charles Daniel CD Segal Kristy K Bryksin Anton A Matsumura Ichiro I
Applied and environmental microbiology 20111021 1
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in this ...[more]
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