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Covariation of major and minor viral capsid proteins in norovirus genogroup II genotype 4 strains.

ABSTRACT: We report sequence hypervariability in the viral protein 1 (VP1) interaction domain of VP2 in the norovirus (NoV) genogroup II genotype 4 (GII.4) lineage on 3 levels: (i) the global evolution of pandemic/epidemic strains from the mid-1970s through post-2006, (ii) the local emergence of an epidemic strain, and (iii) an immunocompromised patient chronically shedding NoV. When a quantitative yeast two-hybrid assay was used, VP2 was found to interact with VP1 in a time-ordered, strain-dependent manner among 3 NoV GII.4 strains. Our findings suggest that VP1 and VP2 may covary in virus evolution and that sequence hypervariability of VP2 may be functionally driven. Further investigations are warranted.

SUBMITTER: Chan MC

PROVIDER: S-EPMC3255813 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Covariation of major and minor viral capsid proteins in norovirus genogroup II genotype 4 strains.

Chan Martin Chi-Wai MC Lee Nelson N Ho Wing-Shan WS Law Carmen Oi-Kwan CO Lau Terrence Chi-Kong TC Tsui Stephen Kwok-Wing SK Sung Joseph Jao-Yiu JJ

Journal of virology 20111109 2

We report sequence hypervariability in the viral protein 1 (VP1) interaction domain of VP2 in the norovirus (NoV) genogroup II genotype 4 (GII.4) lineage on 3 levels: (i) the global evolution of pandemic/epidemic strains from the mid-1970s through post-2006, (ii) the local emergence of an epidemic strain, and (iii) an immunocompromised patient chronically shedding NoV. When a quantitative yeast two-hybrid assay was used, VP2 was found to interact with VP1 in a time-ordered, strain-dependent mann ...[more]

PMID: 22072750

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