Transcriptomic analysis in diabetic nephropathy of streptozotocin-induced diabetic rats.

ABSTRACT: Diabetic nephropathy (DN) is a major complication of diabetes and is caused by an imbalance in the expression of certain genes that activate or inhibit vital cellular functions of kidney. Despite several recent advances, the pathogenesis of DN remains far from clear, suggesting the need to carry out studies identifying molecular aspects, such as gene expression, that could play a key role in the development of DN. There are several techniques to analyze transcriptome in living organisms. In this study, the suppression subtractive hybridization (SSH) method was used to generate up- and down-regulated subtracted cDNA libraries in the kidney of streptozotocin (STZ)-induced diabetic rats. Northern-blot analysis was used to confirm differential expression ratios from the obtained SSH clones to

SUBMITTER: Lomas-Soria C 

PROVIDER: S-EPMC3257079 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature