Accurate RT-qPCR gene expression analysis on cell culture lysates.
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ABSTRACT:
SUBMITTER: Van Peer G
PROVIDER: S-EPMC3257325 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Accurate RT-qPCR gene expression analysis on cell culture lysates.
Van Peer Gert G Mestdagh Pieter P Vandesompele Jo J
Scientific reports 20120113
Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of large numbers of samples. An approach in which cDNA synthesis is carried out on crude cell lysates instead of on purified RNA samples can offer a fast and straightforward alternative. Here, we evaluate such an approach, benchmarking Ambion’s Cells-to ...[more]
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