Ontology highlight
ABSTRACT:
SUBMITTER: Whited CA
PROVIDER: S-EPMC3257399 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20111207 1
We have investigated the kinetics of NO escape from Geobacillus stearothermophilus nitric oxide synthase (gsNOS). Previous work indicated that NO release was gated at position 223 in mammalian enzymes; our kinetics experiments include mutants at that position along with measurements on the wild type enzyme. Employing stopped-flow UV-vis methods, reactions were triggered by mixing a reduced enzyme/N-hydroxy-l-arginine complex with an aerated buffer solution. NO release kinetics were obtained for ...[more]