Ontology highlight
ABSTRACT:
SUBMITTER: Altelaar AF
PROVIDER: S-EPMC3258627 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Altelaar A F Maarten AF Navarro Danny D Boekhorst Jos J van Breukelen Bas B Snel Berend B Mohammed Shabaz S Heck Albert J R AJ
Proceedings of the National Academy of Sciences of the United States of America 20111222 2
Mass spectrometry (MS)-based proteome analysis relies heavily on the presence of complete protein databases. Such a strategy is extremely powerful, albeit not adequate in the analysis of unpredicted postgenome events, such as posttranslational modifications, which exponentially increase the search space. Therefore, it is of interest to explore "database-free" approaches. Here, we sampled the ostrich and human proteomes with a method facilitating de novo sequencing, utilizing the protease Lys-N i ...[more]