Ontology highlight
ABSTRACT:
SUBMITTER: Jishage M
PROVIDER: S-EPMC3259531 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Jishage Miki M Malik Sohail S Wagner Ulrich U Uberheide Beatrix B Ishihama Yasushi Y Hu Xiaopeng X Chait Brian T BT Gnatt Averell A Ren Bing B Roeder Robert G RG
Molecular cell 20120101 1
Pol II(G) is a distinct form of RNA polymerase II that contains the tightly associated Gdown1 polypeptide (encoded by POLR2M). Unlike Pol II, Pol II(G) is highly dependent upon Mediator for robust activator-dependent transcription in a biochemically defined in vitro system. Here, in vitro studies show that Gdown1 competes with TFIIF for binding to the RPB1 and RPB5 subunits of Pol II, thereby inhibiting an essential function of TFIIF in preinitiation complex assembly, but also that Mediator can ...[more]