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ABSTRACT:
SUBMITTER: Puthenpurackal Narayanan S
PROVIDER: S-EPMC3260745 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Puthenpurackal Narayanan Sunilkumar S Maeno Akihiro A Matsuo Hiroshi H Oda Masayuki M Morii Hisayuki H Akasaka Kazuyuki K
Biophysical journal 20120101 2
We studied conformational fluctuations of the transcription factor c-Myb R2 subdomain (52 residues with three Trp) at high pressure and low temperature (5°C) using two different spectroscopic methods, Trp fluorescence and (1)H NMR, on its chemically stable mutant C130I (pseudo-wild-type (WT(S))), which has a large internal cavity. As pressure was increased from 3 to 300 MPa, the Trp fluorescence λ(max) of WT(S) shifted from 342 to ∼355 nm, clearly showing that the three Trp rings become fully ex ...[more]