Ontology highlight
ABSTRACT:
SUBMITTER: Schlacher K
PROVIDER: S-EPMC3261725 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Schlacher Katharina K Christ Nicole N Siaud Nicolas N Egashira Akinori A Wu Hong H Jasin Maria M
Cell 20110501 4
Breast cancer suppressor BRCA2 is critical for maintenance of genomic integrity and resistance to agents that damage DNA or collapse replication forks, presumably through homology-directed repair of double-strand breaks (HDR). Using single-molecule DNA fiber analysis, we show here that nascent replication tracts created before fork stalling with hydroxyurea are degraded in the absence of BRCA2 but are stable in wild-type cells. BRCA2 mutational analysis reveals that a conserved C-terminal site i ...[more]