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Human-like PB2 627K influenza virus polymerase activity is regulated by importin-?1 and -?7.


ABSTRACT: Influenza A viruses may cross species barriers and transmit to humans with the potential to cause pandemics. Interplay of human- (PB2 627K) and avian-like (PB2 627E) influenza polymerase complexes with unknown host factors have been postulated to play a key role in interspecies transmission. Here, we have identified human importin-? isoforms (?1 and ?7) as positive regulators of human- but not avian-like polymerase activity. Human-like polymerase activity correlated with efficient recruitment of ?1 and ?7 to viral ribonucleoprotein complexes (vRNPs) without affecting subcellular localization. We also observed that human-like influenza virus growth was impaired in ?1 and ?7 downregulated human lung cells. Mice lacking ?7 were less susceptible to human- but not avian-like influenza virus infection. Thus, ?1 and ?7 are positive regulators of human-like polymerase activity and pathogenicity beyond their role in nuclear transport.

SUBMITTER: Hudjetz B 

PROVIDER: S-EPMC3262014 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Human-like PB2 627K influenza virus polymerase activity is regulated by importin-α1 and -α7.

Hudjetz Ben B   Gabriel Gülsah G  

PLoS pathogens 20120119 1


Influenza A viruses may cross species barriers and transmit to humans with the potential to cause pandemics. Interplay of human- (PB2 627K) and avian-like (PB2 627E) influenza polymerase complexes with unknown host factors have been postulated to play a key role in interspecies transmission. Here, we have identified human importin-α isoforms (α1 and α7) as positive regulators of human- but not avian-like polymerase activity. Human-like polymerase activity correlated with efficient recruitment of  ...[more]

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