Unknown

Dataset Information

0

Human-like PB2 627K influenza virus polymerase activity is regulated by importin-?1 and -?7.


ABSTRACT: Influenza A viruses may cross species barriers and transmit to humans with the potential to cause pandemics. Interplay of human- (PB2 627K) and avian-like (PB2 627E) influenza polymerase complexes with unknown host factors have been postulated to play a key role in interspecies transmission. Here, we have identified human importin-? isoforms (?1 and ?7) as positive regulators of human- but not avian-like polymerase activity. Human-like polymerase activity correlated with efficient recruitment of ?1 and ?7 to viral ribonucleoprotein complexes (vRNPs) without affecting subcellular localization. We also observed that human-like influenza virus growth was impaired in ?1 and ?7 downregulated human lung cells. Mice lacking ?7 were less susceptible to human- but not avian-like influenza virus infection. Thus, ?1 and ?7 are positive regulators of human-like polymerase activity and pathogenicity beyond their role in nuclear transport.

SUBMITTER: Hudjetz B 

PROVIDER: S-EPMC3262014 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Human-like PB2 627K influenza virus polymerase activity is regulated by importin-α1 and -α7.

Hudjetz Ben B   Gabriel Gülsah G  

PLoS pathogens 20120119 1


Influenza A viruses may cross species barriers and transmit to humans with the potential to cause pandemics. Interplay of human- (PB2 627K) and avian-like (PB2 627E) influenza polymerase complexes with unknown host factors have been postulated to play a key role in interspecies transmission. Here, we have identified human importin-α isoforms (α1 and α7) as positive regulators of human- but not avian-like polymerase activity. Human-like polymerase activity correlated with efficient recruitment of  ...[more]

Similar Datasets

| S-EPMC4359673 | biostudies-literature
| S-EPMC6185888 | biostudies-literature
| S-EPMC3060497 | biostudies-literature
| S-EPMC2222953 | biostudies-literature
| S-EPMC3753988 | biostudies-literature
| S-EPMC2603332 | biostudies-literature
| S-EPMC10006187 | biostudies-literature
| S-EPMC5355620 | biostudies-literature
| S-EPMC2651239 | biostudies-other
| S-EPMC4505657 | biostudies-literature