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Yeast one-hybrid assays for gene-centered human gene regulatory network mapping.

ABSTRACT: Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

SUBMITTER: Reece-Hoyes JS

PROVIDER: S-EPMC3263363 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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Yeast one-hybrid assays for gene-centered human gene regulatory network mapping.

Reece-Hoyes John S JS Barutcu A Rasim AR McCord Rachel Patton RP Jeong Jun Seop JS Jiang Lizhi L MacWilliams Andrew A Yang Xinping X Salehi-Ashtiani Kourosh K Hill David E DE Blackshaw Seth S Zhu Heng H Dekker Job J Walhout Albertha J M AJ

Nature methods 20111030 12

Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors. ...[more]

PMID: 22037702

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