Ontology highlight
ABSTRACT:
SUBMITTER: Musiyenko A
PROVIDER: S-EPMC3263383 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Musiyenko Alla A Bitko Vira V Barik Sailen S
Journal of molecular medicine (Berlin, Germany) 20070310 7
Stable RNA interference (RNAi) is commonly achieved by recombinant expression of short hairpin RNA (shRNA). To generate virus-resistant cell lines, we cloned a shRNA cassette against the phosphoprotein gene of respiratory syncytial virus (RSV) into a polIII-driven plasmid vector. Analysis of individual stable transfectants showed a spectrum of RSV resistance correlating with the levels of shRNA expressed from different chromosomal locations. Interestingly, resistance in a minority of clones was ...[more]