Identification of Escherichia coli genes associated with urinary tract infections.
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ABSTRACT:
SUBMITTER: Mao BH
PROVIDER: S-EPMC3264149 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Publications
Identification of Escherichia coli genes associated with urinary tract infections.
Mao Bin-Hsu BH Chang Yung-Fu YF Scaria Joy J Chang Chih-Ching CC Chou Li-Wei LW Tien Ni N Wu Jiunn-Jong JJ Tseng Chin-Chung CC Wang Ming-Cheng MC Chang Chao-Chin CC Hsu Yuan-Man YM Teng Ching-Hao CH
Journal of clinical microbiology 20111109 2
Escherichia coli is the most common cause of urinary tract infections (UTIs). E. coli genes epidemiologically associated with UTIs are potentially valuable in developing strategies for treating and/or preventing such infections as well as differentiating uropathogenic E. coli from nonuropathogenic E. coli. To identify E. coli genes associated with UTIs in humans, we combined microarray-based and PCR-based analyses to investigate different E. coli source groups derived from feces of healthy human ...[more]
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Project description:One hundred and forty patients with symptomatic urinary tract infection were investigated for serotyping and enterotoxin production. Escherichia coli constituted 63 per cent of the 119 culture positive cases followed by Klebsiella spp and Proteus spp. E. coli serotype O5 was the most prevalent (29.3%) of the typable strains followed by O17 and O25. Out of the 75 E. coli strains, 30 (40%) were enterotoxin producers. Four per cent produced both heat-stable enterotoxin and heat-labile enterotoxin, 21.3 per cent produced only heat stable, whereas 13.3 per cent produced only heat labile toxin. E. coli serotype O5 was the commonest enterotoxin producer (45.5%), followed by rough strains (52.9%) and untypable strains (30.7%). Other ET producing strains were O1, O25, O55, O57 and O60. The enterotoxin production of the most prevalent serotype O5 possibly contributes as a virulence marker in UTI cases.
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