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Novel microsphere-based method for detection and typing of 46 mucosal human papillomavirus types.

ABSTRACT: We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology. Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens. The method was validated with direct sequencing and the Roche Linear Array genotyping method.

SUBMITTER: Zubach V

PROVIDER: S-EPMC3264170 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Novel microsphere-based method for detection and typing of 46 mucosal human papillomavirus types.

Zubach Vanessa V Smart Gerry G Ratnam Samuel S Severini Alberto A

Journal of clinical microbiology 20111123 2

We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology. Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens. The method was validated with direct sequencing ...[more]

PMID: 22116162

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