Ontology highlight
ABSTRACT:
SUBMITTER: Pashkova N
PROVIDER: S-EPMC3266742 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Pashkova Natasha N Gakhar Lokesh L Winistorfer Stanley C SC Yu Liping L Ramaswamy S S Piper Robert C RC
Molecular cell 20101101 3
WD40-repeat β-propellers are found in a wide range of proteins involved in distinct biological activities. We define a large subset of WD40 β-propellers as a class of ubiquitin-binding domains. Using the β-propeller from Doa1/Ufd3 as a paradigm, we find the conserved top surface of the Doa1 β-propeller binds the hydrophobic patch of ubiquitin centered on residues I44, L8, and V70. Mutations that disrupt ubiquitin binding abrogate Doa1 function, demonstrating the importance of this interaction. W ...[more]