Distance and helical phase dependence of synergistic transcription activation in cis-regulatory module.

ABSTRACT: Deciphering of the spatial and stereospecific constraints on synergistic transcription activation mediated between activators bound to cis-regulatory elements is important for understanding gene regulation and remains largely unknown. It has been commonly believed that two activators will activate transcription most effectively when they are bound on the same face of DNA double helix and within a boundary distance from the transcription initiation complex attached to the TATA box. In this work, we studied the spatial and stereospecific constraints on activation by multiple copies of bound model activators using a series of engineered relative distances and stereospecific orientations. We observed that multiple copies of the activators GAL4-VP16 and ZEBRA bound to engineered promoters activ

SUBMITTER: Huang Q 

PROVIDER: S-EPMC3267773 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature