Structure and stability of Gyuba, a ?-lactoglobulin chimera.
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ABSTRACT:
SUBMITTER: Ohtomo H
PROVIDER: S-EPMC3267951 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Publications
Structure and stability of Gyuba, a β-lactoglobulin chimera.
Ohtomo Hideaki H Konuma Tsuyoshi T Utsunoiya Hiroko H Tsuge Hideaki H Ikeguchi Masamichi M
Protein science : a publication of the Protein Society 20110922 11
β-lactoglobulin (LG) contains nine β-strands (strands A-I) and one α-helix. Strands A-H form a β-barrel. At neutral pH, equine LG (ELG) is monomeric, whereas bovine LG (BLG) is dimeric, and the I-strands of its two subunits form an intermolecular β-sheet. We previously constructed a chimeric ELG in which the sequence of the I-strand was replaced with that of BLG. This chimera did not dimerize. For this study, we constructed the new chimera we call Gyuba (which means cow and horse in Japanese). T ...[more]
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