Ontology highlight
ABSTRACT:
SUBMITTER: Nguyen BD
PROVIDER: S-EPMC3268281 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Nguyen Bidong D BD Valdivia Raphael H RH
Proceedings of the National Academy of Sciences of the United States of America 20120109 4
Chlamydia trachomatis, a pathogen responsible for diseases of significant clinical and public health importance, remains poorly characterized because of its intractability to routine molecular genetic manipulation. We have developed a combinatorial approach to rapidly generate a comprehensive library of genetically defined mutants. Chemical mutagenesis, coupled with whole-genome sequencing (WGS) and a system for DNA exchange within infected cells, was used to generate Chlamydia mutants with dist ...[more]