Ontology highlight
ABSTRACT:
SUBMITTER: Raskatov JA
PROVIDER: S-EPMC3268328 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Raskatov Jevgenij A JA Meier Jordan L JL Puckett James W JW Yang Fei F Ramakrishnan Parameswaran P Dervan Peter B PB
Proceedings of the National Academy of Sciences of the United States of America 20111227 4
Nuclear factor κB (NF-κB) is a transcription factor that regulates various aspects of immune response, cell death, and differentiation as well as cancer. In this study we introduce the Py-Im polyamide 1 that binds preferentially to the sequences 5'-WGGWWW-3' and 5'GGGWWW-3'. The compound is capable of binding to κB sites and reducing the expression of various NF-κB-driven genes including IL6 and IL8 by qRT-PCR. Chromatin immunoprecipitation experiments demonstrate a reduction of p65 occupancy wi ...[more]