Ontology highlight
ABSTRACT:
SUBMITTER: Wang S
PROVIDER: S-EPMC3268516 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Wang Shanzhi S Lim Jihyeon J Thomas Keisha K Yan Funing F Angeletti Ruth H RH Schramm Vern L VL
Journal of the American Chemical Society 20120112 3
An enzyme-stabilized nucleophilic water molecule has been implicated at the transition state of Escherichia coli methylthioadenosine nucleosidase (EcMTAN) by transition state analysis and crystallography. We analyzed the EcMTAN mass in complex with a femtomolar transition state analogue to determine whether the inhibitor and nucleophilic water could be detected in the gas phase. EcMTAN-inhibitor and EcMTAN-inhibitor-nucleophilic water complexes were identified by high-resolution mass spectrome ...[more]