Ontology highlight
ABSTRACT:
SUBMITTER: Ozaki S
PROVIDER: S-EPMC3271224 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Biotechnology letters 20111103 3
A glucosyltransferase (GT) of Phytolacca americana (PaGT3) was expressed in Escherichia coli and purified for the synthesis of two O-β-glucoside products of trans-resveratrol. The reaction was moderately regioselective with a ratio of 4'-O-β-glucoside: 3-O-β-glucoside at 10:3. We used not only the purified enzyme but also the E. coli cells containing the PaGT3 gene for the synthesis of glycoconjugates. E. coli cell cultures also have other advantages, such as a shorter incubation time compared w ...[more]