Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA.
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ABSTRACT:
SUBMITTER: Ayora S
PROVIDER: S-EPMC327168 | biostudies-literature | 2004 Jan
REPOSITORIES: biostudies-literature
Publications
Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA.
Ayora Silvia S Carrasco Begoña B Doncel-Perez Ernesto E Lurz Rudi R Alonso Juan C JC
Proceedings of the National Academy of Sciences of the United States of America 20031230 2
Bacillus subtilis RecU protein is involved in homologous recombination, DNA repair, and chromosome segregation. Purified RecU binds preferentially to three- and four-strand junctions when compared to single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) ( approximately 10- and approximately 40-fold lower efficiency, respectively). RecU cleaves mobile four-way junctions but fails to cleave a linear dsDNA with a putative cognate site, a finding consistent with a similar genetic defect observ ...[more]
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