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ABSTRACT:
SUBMITTER: Dempsey GT
PROVIDER: S-EPMC3272503 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Dempsey Graham T GT Vaughan Joshua C JC Chen Kok Hao KH Bates Mark M Zhuang Xiaowei X
Nature methods 20111106 12
One approach to super-resolution fluorescence imaging uses sequential activation and localization of individual fluorophores to achieve high spatial resolution. Essential to this technique is the choice of fluorescent probes; the properties of the probes, including photons per switching event, on-off duty cycle, photostability and number of switching cycles, largely dictate the quality of super-resolution images. Although many probes have been reported, a systematic characterization of the prope ...[more]