Ontology highlight
ABSTRACT:
SUBMITTER: Mack ET
PROVIDER: S-EPMC3272676 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Mack Eric T ET Snyder Phillip W PW Perez-Castillejos Raquel R Bilgiçer Başar B Moustakas Demetri T DT Butte Manish J MJ Whitesides George M GM
Journal of the American Chemical Society 20111221 1
This paper describes a synthetic dimer of carbonic anhydrase, and a series of bivalent sulfonamide ligands with different lengths (25 to 69 Å between the ends of the fully extended ligands), as a model system to use in examining the binding of bivalent antibodies to antigens. Assays based on analytical ultracentrifugation and fluorescence binding indicate that this system forms cyclic, noncovalent complexes with a stoichiometry of one bivalent ligand to one dimer. This dimer binds the series of ...[more]