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Discovering novel bile protection systems in Bifidobacterium breve UCC2003 through functional genomics.


ABSTRACT: Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of bile-induced genes with a range of predicted functions. The potential roles of a selection of these bile-inducible genes in bile protection were analyzed following heterologous expression in Lactococcus lactis. Genes encoding three transport systems belonging to the major facilitator superfamily (MFS), Bbr_0838, Bbr_0832, and Bbr_1756, and three ABC-type transporters, Bbr_0406-0407, Bbr_1804-1805, and Bbr_1826-1827, were thus investigated and shown to provide enhanced resistance and survival to bile exposure. This work significantly improves our understanding as to how bifidobacteria respond to and survive bile exposure.

SUBMITTER: Ruiz L 

PROVIDER: S-EPMC3273026 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Discovering novel bile protection systems in Bifidobacterium breve UCC2003 through functional genomics.

Ruiz Lorena L   Zomer Aldert A   O'Connell-Motherway Mary M   van Sinderen Douwe D   Margolles Abelardo A  

Applied and environmental microbiology 20111209 4


Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of bile-induced genes with a range of predicted functions. The potential roles of a selection of these bile-inducible genes in bile protection were analyzed following heterologous expression in Lactococcus lactis.  ...[more]

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