Ontology highlight
ABSTRACT:
SUBMITTER: Van Nieuwerburgh F
PROVIDER: S-EPMC3273786 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Van Nieuwerburgh Filip F Thompson Ryan C RC Ledesma Jessica J Deforce Dieter D Gaasterland Terry T Ordoukhanian Phillip P Head Steven R SR
Nucleic acids research 20111129 3
Standard Illumina mate-paired libraries are constructed from 3- to 5-kb DNA fragments by a blunt-end circularization. Sequencing reads that pass through the junction of the two joined ends of a 3-5-kb DNA fragment are not easy to identify and pose problems during mapping and de novo assembly. Longer read lengths increase the possibility that a read will cross the junction. To solve this problem, we developed a mate-paired protocol for use with Illumina sequencing technology that uses Cre-Lox rec ...[more]