Ontology highlight
ABSTRACT:
SUBMITTER: Swennenhuis JF
PROVIDER: S-EPMC3273791 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Swennenhuis Joost F JF Foulk Brad B Coumans Frank A W FA Terstappen Leon W M M LW
Nucleic acids research 20111128 3
FISH probes are generally made out of BAC clones with genomic DNA containing a variable amount of repetitive DNA that will need to be removed or blocked for FISH analysis. To generate repeat free (RF) Probes without loss in genomic coverage, a random library is made from BAC clones by whole-genome amplification (WGA). Libraries are denatured in the presence of excess C(0)t-1 DNA and allowed to re-anneal followed by digestion of all double-stranded elements by duplex-specific nuclease (DSN). Sele ...[more]