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Homology-directed Fanconi anemia pathway cross-link repair is dependent on DNA replication.

ABSTRACT: Homologous recombination (also termed homology-directed repair, HDR) is a major pathway for the repair of DNA interstrand cross-links (ICLs) in mammalian cells. Cells from individuals with Fanconi anemia (FA) are characterized by extreme ICL sensitivity, but their reported defect in HDR is mild. Here we examined ICL-induced HDR using a GFP reporter and observed a profound defect in ICL-induced HDR in FA cells, but only when the reporter could replicate.

SUBMITTER: Nakanishi K

PROVIDER: S-EPMC3273992 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Homology-directed Fanconi anemia pathway cross-link repair is dependent on DNA replication.

Nakanishi Koji K Cavallo Francesca F Perrouault Loïc L Giovannangeli Carine C Moynahan Mary Ellen ME Barchi Marco M Brunet Erika E Jasin Maria M

Nature structural & molecular biology 20110320 4

Homologous recombination (also termed homology-directed repair, HDR) is a major pathway for the repair of DNA interstrand cross-links (ICLs) in mammalian cells. Cells from individuals with Fanconi anemia (FA) are characterized by extreme ICL sensitivity, but their reported defect in HDR is mild. Here we examined ICL-induced HDR using a GFP reporter and observed a profound defect in ICL-induced HDR in FA cells, but only when the reporter could replicate. ...[more]

PMID: 21423196

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