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Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgaris L.).

ABSTRACT: The complex inheritance of resistance to Cercospora leaf spot (CLS), the most severe fungal foliar disease in sugar beet, was investigated by means of quantitative trait loci (QTL) analysis. Over a three year period, recombinant inbred lines (RILs) of sugar beet (Beta vulgaris L.), generated through a cross between lines resistant ('NK-310mm-O') and susceptible ('NK-184mm-O') to CLS, were field-tested for their resistance to the pathogen. Composite interval mapping (CIM) showed four QTL involved in CLS resistance to be consistently detected. Two resistant QTL (qcr1 on chromosome III, qcr4 on chromosome IX) bearing 'NK-310mm-O' derived alleles promoted resistance. Across 11 investigations, the qcr1 and qcr4 QTL explained approximately 10% and over 20%, respectively, of the variance in the resistance index. Two further QTL (qcr2 on chromosome IV, qcr3 on chromosome VI) bearing 'NK-184mm-O' derived alleles each explained about 10% of the variance. To identify the monogenic effect of the resistance, two QTL derived from 'NK-310mm-O' against the genetic background of 'NK-184mm-O', using molecular markers. The qcr1 and qcr4 were precisely mapped as single QTL, using progenies BC(5)F(1) and BC(2)F(1), respectively. The qcr1 that was located near e11m36-8 had CLS disease severity indices (DSI) about 15% lower than plants homozygous for the 'NK-184mm-O' genotype. As with qcr1, heterozygosis of the qcr4 that was located near e17m47-81 reduced DSI by about 45% compared to homozygosis. These two resistant QTL might be of particular value in marker-assisted selection (MAS) programs in CLS resistance progression.

SUBMITTER: Taguchi K

PROVIDER: S-EPMC3276142 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgaris L.).

Taguchi Kazunori K Kubo Tomohiko T Takahashi Hiroyuki H Abe Hideyuki H

G3 (Bethesda, Md.) 20110901 4

The complex inheritance of resistance to Cercospora leaf spot (CLS), the most severe fungal foliar disease in sugar beet, was investigated by means of quantitative trait loci (QTL) analysis. Over a three year period, recombinant inbred lines (RILs) of sugar beet (Beta vulgaris L.), generated through a cross between lines resistant ('NK-310mm-O') and susceptible ('NK-184mm-O') to CLS, were field-tested for their resistance to the pathogen. Composite interval mapping (CIM) showed four QTL involved ...[more]

PMID: 22384339

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Project description:Cercospora leaf spot, caused by the fungal pathogen Cercospora beticola, is the most destructive foliar disease of sugar beet worldwide. This review discusses C. beticola genetics, genomics, and biology and summarizes our current understanding of the molecular interactions that occur between C. beticola and its sugar beet host. We highlight the known virulence arsenal of C. beticola as well as its ability to overcome currently used disease management strategies. Finally, we discuss future prospects for the study and management of C. beticola infections in the context of newly employed molecular tools to uncover additional information regarding the biology of this pathogen.TaxonomyCercospora beticola Sacc.; Kingdom Fungi, Phylum Ascomycota, Class Dothideomycetes, Order Capnodiales, Family Mycosphaerellaceae, Genus Cercospora.Host rangeWell-known pathogen of sugar beet (Beta vulgaris subsp. vulgaris) and most species of the Beta genus. Reported as pathogenic on other members of the Chenopodiaceae (e.g., lamb's quarters, spinach) as well as members of the Acanthaceae (e.g., bear's breeches), Apiaceae (e.g., Apium), Asteraceae (e.g., chrysanthemum, lettuce, safflower), Brassicaceae (e.g., wild mustard), Malvaceae (e.g., Malva), Plumbaginaceae (e.g., Limonium), and Polygonaceae (e.g., broad-leaved dock) families.Disease symptomsLeaves infected with C. beticola exhibit circular lesions that are coloured tan to grey in the centre and are often delimited by tan-brown to reddish-purple rings. As disease progresses, spots can coalesce to form larger necrotic areas, causing severely infected leaves to wither and die. At the centre of these spots are black spore-bearing structures (pseudostromata). Older leaves often show symptoms first and younger leaves become infected as the disease progresses.ManagementApplication of a mixture of fungicides with different modes of action is currently performed although elevated resistance has been documented in most employed fungicide classes. Breeding for high-yielding cultivars with improved host resistance is an ongoing effort and prudent cultural practices, such as crop rotation, weed host management, and cultivation to reduce infested residue levels, are widely used to manage disease. USEFUL WEBSITE: https://www.ncbi.nlm.nih.gov/genome/11237?genome_assembly_id=352037.

Project description:BACKGROUND: Gray leaf spot (GLS) is a globally important foliar disease of maize. Cercospora zeina, one of the two fungal species that cause the disease, is prevalent in southern Africa, China, Brazil and the eastern corn belt of the USA. Identification of QTL for GLS resistance in subtropical germplasm is important to support breeding programmes in developing countries where C.?zeina limits production of this staple food crop. RESULTS: A maize RIL population (F7:S6) from a cross between CML444 and SC Malawi was field-tested under GLS disease pressure at five field sites over three seasons in KwaZulu-Natal, South Africa. Thirty QTL identified from eleven field trials (environments) were consolidated to seven QTL for GLS resistance based on their expression in at least two environments and location in the same core maize bins. Four GLS resistance alleles were derived from the more resistant parent CML444 (bin 1.10, 4.08, 9.04/9.05, 10.06/10.07), whereas the remainder were from SC Malawi (bin 6.06/6.07, 7.02/7.03, 9.06). QTLs in bin 4.08 and bin 6.06/6.07 were also detected as joint QTLs, each explained more than 11% of the phenotypic variation, and were identified in four and seven environments, respectively. Common markers were used to allocate GLS QTL from eleven previous studies to bins on the IBM2005 map, and GLS QTL "hotspots" were noted. Bin 4.08 and 7.02/7.03 GLS QTL from this study overlapped with hotspots, whereas the bin 6.06/6.07 and bin 9.06 QTLs appeared to be unique. QTL for flowering time (bin 1.07, 4.09) in this population did not correspond to QTL for GLS resistance. CONCLUSIONS: QTL mapping of a RIL population from the subtropical maize parents CML444 and SC Malawi identified seven QTL for resistance to gray leaf spot disease caused by C.?zeina. These QTL together with QTL from eleven studies were allocated to bins on the IBM2005 map to provide a basis for comparison. Hotspots of GLS QTL were identified on chromosomes one, two, four, five and seven, with QTL in the current study overlapping with two of these. Two QTL from this study did not overlap with previously reported QTL.

Dataset Information

Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgaris L.).

Publications

Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgaris L.).

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