Ontology highlight
ABSTRACT:
SUBMITTER: Zhou H
PROVIDER: S-EPMC3281001 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Zhou Hongwei H DeLoid Glen G Browning Erica E Gregory David J DJ Tan Fengxiao F Bedugnis Alice S AS Imrich Amy A Koziel Henry H Kramnik Igor I Lu Quan Q Kobzik Lester L
PloS one 20120216 2
Interferon-gamma (IFN-γ) inhibits intracellular replication of Francisella tularensis in human monocyte-derived macrophages (HMDM) and in mice, but the mechanisms of this protective effect are poorly characterized. We used genome-wide RNA interference (RNAi) screening in the human macrophage cell line THP-1 to identify genes that mediate the beneficial effects of IFN-γ on F. tularensis infection. A primary screen identified ∼200 replicated candidate genes. These were prioritized according to mRN ...[more]