Ontology highlight
ABSTRACT:
SUBMITTER: Ymele-Leki P
PROVIDER: S-EPMC3281070 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Ymele-Leki Patrick P Cao Shugeng S Sharp Jared J Lambert Kathleen G KG McAdam Alexander J AJ Husson Robert N RN Tamayo Giselle G Clardy Jon J Watnick Paula I PI
PloS one 20120216 2
Due to the inexorable invasion of our hospitals and communities by drug-resistant bacteria, there is a pressing need for novel antibacterial agents. Here we report the development of a sensitive and robust but low-tech and inexpensive high-throughput metabolic screen for novel antibiotics. This screen is based on a colorimetric assay of pH that identifies inhibitors of bacterial sugar fermentation. After validation of the method, we screened over 39,000 crude extracts derived from organisms that ...[more]