Using transcriptome sequencing to identify mechanisms of drug action and resistance.
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ABSTRACT:
SUBMITTER: Wacker SA
PROVIDER: S-EPMC3281560 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Publications
Using transcriptome sequencing to identify mechanisms of drug action and resistance.
Wacker Sarah A SA Houghtaling Benjamin R BR Elemento Olivier O Kapoor Tarun M TM
Nature chemical biology 20120212 3
Determining mechanisms of drug action in human cells remains a major challenge. Here we describe an approach in which multiple-drug-resistant clones are isolated and transcriptome sequencing is used to find mutations in each clone. Further analysis of mutations common to more than one clone can identify a drug's physiological target and indirect resistance mechanisms, as indicated by our proof-of-concept studies of the cytotoxic anticancer drugs BI 2536 and bortezomib. ...[more]
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