Browse
Submit Data
Databases
API
Help

Dataset Information

26 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stimuli-responsive smart gels realized via modular protein design.

ABSTRACT: Smart gels have a variety of applications, including tissue engineering and controlled drug delivery. Here we present a modular, bottom-up approach that permits the creation of protein-based smart gels with encoded morphology, functionality, and responsiveness to external stimuli. The properties of these gels are encoded by the proteins from which they are synthesized. In particular, the strength and density of the network of intermolecular cross-links are specified by the interactions of the gels' constituent protein modules with their cognate peptide ligands. Thus, these gels exhibit stimuli-responsive assembly and disassembly, dissolving (or gelling) under conditions that weaken (or strengthen) the protein-peptide interaction. We further demonstrate that such gels can encapsulate and release both proteins and small molecules and that their rheological properties are well suited for biomedical applications.

SUBMITTER: Grove TZ

PROVIDER: S-EPMC3282109 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Stimuli-responsive smart gels realized via modular protein design.

Grove Tijana Z TZ Osuji Chinedum O CO Forster Jason D JD Dufresne Eric R ER Regan Lynne L

Journal of the American Chemical Society 20101001 40

Smart gels have a variety of applications, including tissue engineering and controlled drug delivery. Here we present a modular, bottom-up approach that permits the creation of protein-based smart gels with encoded morphology, functionality, and responsiveness to external stimuli. The properties of these gels are encoded by the proteins from which they are synthesized. In particular, the strength and density of the network of intermolecular cross-links are specified by the interactions of the ge ...[more]

PMID: 20860358

Similar Datasets

Design of modular dual enzyme-responsive peptides.

Project description:Dual enzyme-responsive peptides were synthesized by masking the ?-amine of lysine with various enzyme substrates. Enzymatic cleavage of these sequences unmasked the ?-amine, allowing for further digestion by a second enzyme, which was monitored colorimetrically. This modular peptide design should provide substrates for a large combination of clinically relevant enzymes.

| S-EPMC5963286 | biostudies-literature

Smart Nanoscale Drug Delivery Platforms from Stimuli-Responsive Polymers and Liposomes.

Project description:Since the 1960's, stimuli-responsive polymers have been utilized as functional soft materials for biological applications such as the triggered-release delivery of biologically active cargos. Over the same period, liposomes have been explored as an alternative drug delivery system with potentials to decrease the toxic side effects often associated with conventional small-molecule drugs. However, the lack of drug-release triggers and the instability of bare liposomes often limit their practical applications, causing short circulation time and low therapeutic efficacy. This perspective article highlights recent work in integrating these two materials together to achieve a targetable, triggerable nanoscale platform that fulfills all the characteristics of a near-ideal drug delivery system. Through a drop-in, post-synthesis modification strategy, a network of stimuli-responsive polymers can be integrated onto the surface of liposomes to form polymer-caged nanobins, a multifunctional nanoscale delivery platform that allows for multi-drug loading, targeted delivery, triggered drug-release, and theranostic capabilities.

| S-EPMC5552073 | biostudies-other

Reversible Dual-Stimuli-Responsive Chromic Transparent Wood Biocomposites for Smart Window Applications.

Project description:Transparent wood (TW)-based composites are of significant interest for smart window applications. In this research, we demonstrate a facile dual-stimuli-responsive chromic TW where optical properties are reversibly controlled in response to changes in temperature and UV-radiation. For this functionality, bleached wood was impregnated with solvent-free thiol and ene monomers containing chromic components, consisting of a mixture of thermo- and photoresponsive chromophores, and was then UV-polymerized. Independent optical properties of individual chromic components were retained in the compositional mixture. This allowed to enhance the absolute optical transmission to 4 times above the phase change temperature. At the same time, the transmission at 550 nm could be reduced 11-77%, on exposure to UV by changing the concentration of chromic components. Chromic components were localized inside the lumen of the wood structure, and durable reversible optical properties were demonstrated by multiple cycling testing. In addition, the chromic TW composites showed reversible energy absorption capabilities for heat storage applications and demonstrated an enhancement of 64% in the tensile modulus as compared to a native thiol-ene polymer. This study elucidates the polymerization process and effect of chromic components distribution and composition on the material's performance and perspectives toward the development of smart photoresponsive windows with energy storage capabilities.

| S-EPMC7821267 | biostudies-literature

Role of Mechanical Factors in Applications of Stimuli-Responsive Polymer Gels - Status and Prospects.

Project description:Due to their unique characteristics such as multifold change of volume in response to minute change in the environment, resemblance of soft biological tissues, ability to operate in wet environments, and chemical tailorability, stimuli responsive gels represent a versatile and very promising class of materials for sensors, muscle-type actuators, biomedical applications, and autonomous intelligent structures. Success of these materials in practical applications largely depends on their ability to fulfill application-specific mechanical requirements. This article provides an overview of recent application-driven development of covalent polymer gels with special emphasis on the relevant mechanical factors and properties. A short account of mechanisms of gel swelling and mechanical characteristics of importance to stimuli-responsive gels is presented. The review highlights major barriers for wider application of these materials and discusses latest advances and potential future directions toward overcoming these barriers, including interpenetrating networks, homogeneous networks, nanocomposites, and nanofilamentary gels.

| S-EPMC5365095 | biostudies-literature

Smart vaults: thermally-responsive protein nanocapsules.

Project description:Synthetic modification of a recombinant protein cage called a vault with stimuli-responsive smart polymers provides access to a new class of biohybrid materials; the polymer nanocapsules retain the structure of the protein cage and exhibit the responsive nature of the polymer. Vaults are naturally occurring ubiquitous ribonucleoprotein particles 41 × 41 × 72.5 nm composed of a protein shell enclosing multiple copies of two proteins and multiple copies of one or more small untranslated RNAs. Recombinant vaults are structurally identical but lack the vault content. Poly(N-isopropylacrylamide) (pNIPAAm), a polymer responsive to heat, was conjugated to recombinant vaults that were composed of ~78 copies of the major vault protein (MVP) modified to contain a cysteine rich region at the N-terminus (CP-MVP). The polymer was synthesized using reversible addition-fragmentation chain transfer (RAFT) polymerization to have a dansyl group at the alpha end and modified to have a thiol-reactive pyridyl disulfide at the omega end, which readily coupled to CP-MVP vaults. The resulting vault nanocapsules underwent reversible aggregation upon heating above the lower critical solution temperature (LCST) of the polymer as determined by electron microscopy (EM), dynamic light scattering experiments, and UV-vis turbidity analysis. The vault structure remained entirely intact throughout the phase transition; suggesting its use in a myriad of biomedical and biotechnology applications.

| S-EPMC3991814 | biostudies-literature

Smart Material Hydrogel Transfer Devices Fabricated with Stimuli-Responsive Silk-Elastin-Like Proteins.

Project description:Three-dimensional organoid tissue culture models are a promising approach for the study of biological processes including diseases. Advances in these tissue culture technologies improve in vitro analysis compared to standard 2D cellular approaches and are more representative of the physiological environment. However, a major challenge associated with organoid systems stems from the laborious processing involved in the analysis of large numbers of organoids. Here the design, characterization, and application of silk-elastin-like protein-based smart carrier arrays for processing organoids is presented. Fabrication of hydrogel-based carrier systems at room temperature result in organized arrays of organoids that maintain tissue culture plate orientation and could be processed simultaneously for histology. The system works by transfer of the organoids to the hydrogel arrays after which the material is subjected to 65 °C to induce hydrogel contraction to secure the organoids, resulting in multisample constructs and allowing for placement on a microscope slide. Histological processing and immunostaining of these arrayed cerebral organoids analyzed within the contracted silk-elastin-like proteins (SELP) show retention of native organoid features compared to controls without the hydrogel carrier system, thus avoiding any artifacts. These SELP carriers present a useful approach for improving efficiency of scaled organoid screening and processing.

| S-EPMC7466789 | biostudies-literature

Making and Breaking of Gels: Stimuli-Responsive Properties of Bis(Pyridyl-N-oxide Urea) Gelators.

Project description:The structural modification of existing supramolecular architecture is an efficient strategy to design and synthesize supramolecular gels with tunable and predictable properties. In this work, we have modified bis(pyridyl urea) compounds with different linkers, namely hexylene and butylene, to their corresponding bis(pyridyl-N-oxide urea). The gelation properties of both the parent and the modified compounds were studied, and the results indicated that modification of the 3-pyridyl moieties to the corresponding 3-pyridyl-N-oxides induced hydrogelation. The stability of the parent and modified compounds were evaluated by sol-gel transition temperature (Tgel) and rheological measurements, and single-crystal X-ray diffraction was used to analyze the solid-state interactions of the gelators. The morphologies of the dried gels were analyzed by scanning electron microscopy (SEM), which revealed that the structural modification did not induce any prominent effect on the gel morphology. The stimuli-responsive behavior of these gels in the presence of salts in DMSO/water was evaluated by rheological experiments, which indicated that the modified compounds displayed enhanced gel strength in most cases. However, the gel network collapsed in the presence of the chloride salts of aluminum(III), zinc(II), copper(II), and cadmium(II). The mechanical strength of the parent gels decreased in the presence of salts, indicating that the structural modification resulted in robust gels in most cases. The modified compounds formed gels below minimum gel concentration in the presence of various salts, indicating salt-induced gelation. These results show the making and breaking ability of the gel network in the presence of external stimuli (salts), which explains the potential of using LMWGs based on N-oxide moieties as stimuli-responsive materials.

| S-EPMC8587056 | biostudies-literature

Smart Nanoformulation Based on Stimuli-Responsive Nanogels and Curcumin: Promising Therapy against Colon Cancer.

Project description:Curcumin (CUR) has gained much attention for its widely reported anticancer effect; however, its clinical use is restricted due to its low water solubility and, consequently, its poor bioavailability. Here, we report on the use of a nanoformulation of CUR with cationic nanogels for colon cancer therapy. Cationic stimuli-sensitive nanogels were prepared using a scale-up polymerization methodology based on surfactant-free emulsion polymerization of N,N'-diethylaminoethyl methacrylate (DEAEM) and poly(ethyleneglycol) methacrylate (PEGMA). The obtained nanogels showed a homogeneous size distribution (from 51 to 162 nm, polydispersity index (PDI) < 0.138) and exhibited a spherical form and core-shell morphology as confirmed by dynamic light scattering and electron microscopy, respectively. Nanogels were responsive to and degradable by variations of pH, temperature, or the redox environment, depending on the cross-linker used in the synthesis. Nanogels cross-linked with bis(acryloyl)cystamine incubated in a buffer (pH 7.4) containing 3 mM glutathione degraded in 60 min, while nanogels cross-linked with a divinylacetal cross-linker degraded in 10 min (pH ? 6). Nanoformulations of nanogels with CUR were stable as tested up to 30 days at physiological conditions. In vitro studies of the human colon cancer cell line (HCT-116) showed a synergistic effect of CUR and the degradable nanogels. Further, in vivo acute cytotoxicity tests of empty nanogels in mice demonstrate their potential as CUR nanocarriers for colon-anticancer therapies.

| S-EPMC7191563 | biostudies-literature

Rhodamine-Installed Polynorbornenes: Molecular Design, Structure, and Stimuli-Responsive Properties.

Project description:The synthesis of a number of tailored architectures of rhodamine dye-norbornene conjugate monomers and corresponding homopolymers derived from them is described. The impact of the monomer architecture on the mechanochromic, photochromic, and thermochromic properties of rhodamine-modified polynorbornenes is reported. Color changes were caused by the reversible interconversion between the "open" and "closed" spirolactam form of the covalently attached dye. Monomers were synthesized in two principle architectures that varied on: (1) the number of polymerizable norbornene groups tethered to a bifunctional rhodamine dye; (2) the presence of flexible methylene spacers between the dye and the polymerizable norbornene groups. Introduction of norbornene groups on each of the two hydroxy groups of a bifunctional rhodamine resulted in a cross-linked polymer that exhibited better mechanochromic, photochromic, and thermochromic properties compared to the corresponding polymer without cross-links, derived from the derivatization of bifunctional rhodamine with only one norbornene. The introduction of flexible methylene spacers between the two polymerizable norbornenes and the dye molecule resulted in a polymeric framework with rapidly reversible color-changing properties upon mechanical or photostimulation. The ideal monomer molecular structure, whereby (1) attaching norbornene on both sides of the rhodamine dye and (2) methylene spacers between the dye and norbornenes on both sides afforded the nonpareil polymer structure that was capable of thermoreversible mechanochromic and photochromic features, and irreversible thermochromic features. These new materials may find utility as multi-stimuli-responsive soft materials.

| S-EPMC8210439 | biostudies-literature

Design of modular autoproteolytic gene switches responsive to anti-coronavirus drug candidates.

Project description:The main (Mpro) and papain-like (PLpro) proteases encoded by SARS-CoV-2 are essential to process viral polyproteins into functional units, thus representing key targets for anti-viral drug development. There is a need for an efficient inhibitor screening system that can identify drug candidates in a cellular context. Here we describe modular, tunable autoproteolytic gene switches (TAGS) relying on synthetic transcription factors that self-inactivate, unless in the presence of coronavirus protease inhibitors, consequently activating transgene expression. TAGS rapidly report the impact of drug candidates on Mpro and PLpro activities with a high signal-to-noise response and a sensitivity matching concentration ranges inhibiting viral replication. The modularity of the TAGS enabled the study of other Coronaviridae proteases, characterization of mutations and multiplexing of gene switches in human cells. Mice implanted with Mpro or PLpro TAGS-engineered cells enabled analysis of the activity and bioavailability of protease inhibitors in vivo in a virus-free setting.

| S-EPMC8609006 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data