Analysis of the Tomato spotted wilt virus ambisense S RNA-encoded hairpin structure in translation.

ABSTRACT:

Background

The intergenic region (IR) of ambisense RNA segments from animal- and plant-infecting (-)RNA viruses functions as a bidirectional transcription terminator. The IR sequence of the Tomato spotted wilt virus (TSWV) ambisense S RNA contains stretches that are highly rich in A-residues and U-residues and is predicted to fold into a stable hairpin structure. The presence of this hairpin structure sequence in the 3' untranslated region (UTR) of TSWV mRNAs implies a possible role in translation.

Methodology/principal findings

To analyse the role of the predicted hairpin structure in translation, various Renilla luciferase constructs containing modified 3' and/or 5' UTR sequences of the TSWV S RNA encoded nucleocapsid (N) gene were analyzed for expression. While good lucif

SUBMITTER: Geerts-Dimitriadou C 

PROVIDER: S-EPMC3283609 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature