Ontology highlight
ABSTRACT:
SUBMITTER: Mukherjee K
PROVIDER: S-EPMC3286871 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Mukherjee Konark K Sharma Manu M Jahn Reinhard R Wahl Markus C MC Südhof Thomas C TC
Science signaling 20100427 119
All known protein kinases, except CASK [calcium/calmodulin (CaM)-activated serine-threonine kinase], require magnesium ions (Mg(2+)) to stimulate the transfer of a phosphate from adenosine 5'-triphosphate (ATP) to a protein substrate. The CaMK (calcium/calmodulin-dependent kinase) domain of CASK shows activity in the absence of Mg(2+); indeed, it is inhibited by divalent ions including Mg(2+). Here, we converted the Mg(2+)-inhibited wild-type CASK kinase (CASK(WT)) into a Mg(2+)-stimulated kinas ...[more]