Dataset Information

F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the actinomycetales.

ABSTRACT: Two classes of F(420)-dependent reductases (FDR-A and FDR-B) that can reduce aflatoxins and thereby degrade them have previously been isolated from Mycobacterium smegmatis. One class, the FDR-A enzymes, has up to 100 times more activity than the other. F(420) is a cofactor with a low reduction potential that is largely confined to the Actinomycetales and some Archaea and Proteobacteria. We have heterologously expressed ten FDR-A enzymes from diverse Actinomycetales, finding that nine can also use F(420)H(2) to reduce aflatoxin. Thus FDR-As may be responsible for the previously observed degradation of aflatoxin in other Actinomycetales. The one FDR-A enzyme that we found not to reduce aflatoxin belonged to a distinct clade (herein denoted FDR-AA), and our subsequent expression and analysis of seven other FDR-AAs from M. smegmatis found that none could reduce aflatoxin. Certain FDR-A and FDR-B enzymes that could reduce aflatoxin also showed activity with coumarin and three furanocoumarins (angelicin, 8-methoxysporalen and imperatorin), but none of the FDR-AAs tested showed any of these activities. The shared feature of the compounds that were substrates was an ?,?-unsaturated lactone moiety. This moiety occurs in a wide variety of otherwise recalcitrant xenobiotics and antibiotics, so the FDR-As and FDR-Bs may have evolved to harness the reducing power of F(420) to metabolise such compounds. Mass spectrometry on the products of the FDR-catalyzed reduction of coumarin and the other furanocoumarins shows their spontaneous hydrolysis to multiple products.

SUBMITTER: Lapalikar GV

PROVIDER: S-EPMC3288000 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the actinomycetales.

Lapalikar Gauri V GV Taylor Matthew C MC Warden Andrew C AC Scott Colin C Russell Robyn J RJ Oakeshott John G JG

PloS one 20120227 2

Two classes of F(420)-dependent reductases (FDR-A and FDR-B) that can reduce aflatoxins and thereby degrade them have previously been isolated from Mycobacterium smegmatis. One class, the FDR-A enzymes, has up to 100 times more activity than the other. F(420) is a cofactor with a low reduction potential that is largely confined to the Actinomycetales and some Archaea and Proteobacteria. We have heterologously expressed ten FDR-A enzymes from diverse Actinomycetales, finding that nine can also us ...[more]

PMID: 22383957

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Dataset Information

F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the actinomycetales.

Publications

F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the actinomycetales.

Similar Datasets

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