Dataset Information

The ?3 chain of laminin is widely but differentially expressed in murine basement membranes: expression and functional studies.

ABSTRACT: Laminins are heterotrimeric extracellular glycoproteins found in, but not confined to, basement membranes (BMs). They are important components in formation of the molecular networks of BMs as well as in cell polarity, cell differentiation and tissue morphogenesis. Each laminin is composed by an ?, a ? and a ? chain. Previous studies have shown that the ?3 chain is partnered with either the ?1 chain (in placenta) or ?2 chain (in the CNS) (Libby et al., 2000). Several studies, including our own, suggested that the ?3 chain is expressed in both apical and basal compartments (Koch et al., 1999; Gersdorff et al., 2005; Yan and Cheng, 2006). This study investigates the expression pattern of the ?3 chain in mouse. We developed three new ?3-reactive antibodies, and we show that the ?3 chain is present in BMs. The distribution pattern is considerably more restricted than that of the ?1 chain and within any tissue there is differential deposition into BM compartments. This is particularly true in the retina and brain, where ?3 is uniquely expressed in a subset of the vascular basement membranes and the pial surface. We used conventional genetic ablation techniques to remove the ?3 chain in mice; unlike other laminin null mice (?5, ?2, ?1 nulls), these mice live a normal lifespan and have only minor abnormalities, the most striking of which are ectopic granule cells in the cerebellum and an apparent increase in capillary branching in the outer retina. These data support the suggestion that the ?3 chain is deposited in BMs and contributes some unique properties to their function, particularly in the nervous system.

SUBMITTER: Li YN

PROVIDER: S-EPMC3288684 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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The γ3 chain of laminin is widely but differentially expressed in murine basement membranes: expression and functional studies.

Li Yong N YN Radner Stephanie S French Margaret M MM Pinzón-Duarte Germán G Daly Gerard H GH Burgeson Robert E RE Koch Manuel M Brunken William J WJ

Matrix biology : journal of the International Society for Matrix Biology 20111224 2

Laminins are heterotrimeric extracellular glycoproteins found in, but not confined to, basement membranes (BMs). They are important components in formation of the molecular networks of BMs as well as in cell polarity, cell differentiation and tissue morphogenesis. Each laminin is composed by an α, a β and a γ chain. Previous studies have shown that the γ3 chain is partnered with either the β1 chain (in placenta) or β2 chain (in the CNS) (Libby et al., 2000). Several studies, including our own, s ...[more]

PMID: 22222602

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Project description:Allergic asthma is one of the most common allergic diseases; however, the mechanisms underlying its development have yet to be fully elucidated. Although allergic diseases are inheritable, genetic variance alone cannot explain the notable increase in the prevalence of allergic diseases over a short period of time in recent decades. Recently, research focus has been shifting to epigenetic factors, such as non‑coding RNAs. Circular RNAs (circRNAs) are involved in the pathogenesis of various diseases. The aim of the present study was to further elucidate the etiology of allergic asthma by analyzing aberrantly expressed circRNAs in a murine asthma model. A mouse model of house dust mite allergen‑induced asthma was established, and the qualified libraries were sequenced using next‑generation sequencing. The expression levels of circRNAs were validated by reverse transcription‑quantitative PCR (RT‑qPCR) analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed for biological pathway classification and enrichment analysis of the aberrantly expressed circRNAs. In addition, the interaction network of the differentially expressed circRNAs and microRNAs (miRNAs) was constructed using Cytoscape. By next‑generation sequencing, a total of 150 circRNAs were revealed to be upregulated and 130 were downregulated in the murine asthma model group compared with in the control group. GO and KEGG analyses demonstrated that the differentially expressed circRNAs were mainly involved in processes such as 'autoimmune disease', 'cell adhesion molecules (CAMs)' and 'endocytosis', among others. The expression levels of six circRNAs, namely three upregulated (circ_0000909, circ_0000629 and circ_0000455) and three downregulated (circ_0001454, circ_0000723 and circ_0001389) circRNAs, were validated by RT‑qPCR. In conclusion, the analyses suggested that circRNAs performed critical functions via endocytosis (such as macrophage endocytosis), cell adhesion molecules and lipid metabolism in allergic asthma. The interaction network revealed that certain miRNAs that may serve a role in asthma could be regulated by the differentially expressed circRNAs.

Dataset Information

The ?3 chain of laminin is widely but differentially expressed in murine basement membranes: expression and functional studies.

Publications

The γ3 chain of laminin is widely but differentially expressed in murine basement membranes: expression and functional studies.

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