Multiplex targeted proteomic assay for biomarker detection in plasma: a pancreatic cancer biomarker case study.

ABSTRACT: Biomarkers are most frequently proteins that are measured in the blood. Their development largely relies on antibody creation to test the protein candidate performance in blood samples of diseased versus nondiseased patients. The creation of such antibody assays has been a bottleneck in biomarker progress due to the cost, extensive time, and effort required to complete the task. Targeted proteomics is an emerging technology that is playing an increasingly important role to facilitate disease biomarker development. In this study, we applied a SRM-based targeted proteomics platform to directly detect candidate biomarker proteins in plasma to evaluate their clinical utility for pancreatic cancer detection. The characterization of these protein candidates used a clinically well-characterized c

SUBMITTER: Pan S 

PROVIDER: S-EPMC3292708 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature