Ontology highlight
ABSTRACT:
SUBMITTER: Friend K
PROVIDER: S-EPMC3293257 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Friend Kyle K Campbell Zachary T ZT Cooke Amy A Kroll-Conner Peggy P Wickens Marvin P MP Kimble Judith J
Nature structural & molecular biology 20120108 2
PUF (Pumilio/FBF) RNA-binding proteins and Argonaute (Ago) miRNA-binding proteins regulate mRNAs post-transcriptionally, each acting through similar, yet distinct, mechanisms. Here, we report that PUF and Ago proteins can also function together in a complex with a core translation elongation factor, eEF1A, to repress translation elongation. Both nematode (Caenorhabditis elegans) and mammalian PUF-Ago-eEF1A complexes were identified, using coimmunoprecipitation and recombinant protein assays. Nem ...[more]