The spectroscopic basis of fluorescence triple correlation spectroscopy.

ABSTRACT: We have developed fluorescence triple correlation spectroscopy (F3CS) as an extension of the widely used fluorescence microscopy technique fluorescence correlation spectroscopy. F3CS correlates three signals at once and provides additional capabilities for the study of systems with complex stoichiometry, kinetic processes, and irreversible reactions. A general theory of F3CS was developed to describe the interplay of molecular dynamics and microscope optics, leading to an analytical function to predict experimental triple correlations of molecules that freely diffuse through the tight focus of the microscope. Experimental correlations were calculated from raw fluorescence data using triple correlation integrals that extend multiple-tau correlation theory to delay times in two dimensions. T

SUBMITTER: Ridgeway WK 

PROVIDER: S-EPMC3293655 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature