Dataset Information

Cd2+ Toxicity to a green alga Chlamydomonas reinhardtii as influenced by its adsorption on TiO2 engineered nanoparticles.

ABSTRACT: In the present study, Cd(2+) adsorption on polyacrylate-coated TiO(2) engineered nanoparticles (TiO(2)-ENs) and its effect on the bioavailability as well as toxicity of Cd(2+) to a green alga Chlamydomonas reinhardtii were investigated. TiO(2)-ENs could be well dispersed in the experimental medium and their pH(pzc) is approximately 2. There was a quick adsorption of Cd(2+) on TiO(2)-ENs and a steady state was reached within 30 min. A pseudo-first order kinetics was found for the time-related changes in the amount of Cd(2+) complexed with TiO(2)-ENs. At equilibrium, Cd(2+) adsorption followed the Langmuir isotherm with the maximum binding capacity 31.9, 177.1, and 242.2 mg/g when the TiO(2)-EN concentration was 1, 10, and 100 mg/l, respectively. On the other hand, Cd(2+) toxicity was alleviated in the presence of TiO(2)-ENs. Algal growth was less suppressed in treatments with comparable total Cd(2+) concentration but more TiO(2)-ENs. However, such toxicity difference disappeared and all the data points could be fitted to a single Logistic dose-response curve when cell growth inhibition was plotted against the free Cd(2+) concentration. No detectable amount of TiO(2)-ENs was found to be associated with the algal cells. Therefore, TiO(2)-ENs could reduce the free Cd(2+) concentration in the toxicity media, which further lowered its bioavailability and toxicity to C. reinhardtii.

SUBMITTER: Yang WW

PROVIDER: S-EPMC3293805 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Cd2+ Toxicity to a green alga Chlamydomonas reinhardtii as influenced by its adsorption on TiO2 engineered nanoparticles.

Yang Wei-Wan WW Miao Ai-Jun AJ Yang Liu-Yan LY

PloS one 20120305 3

In the present study, Cd(2+) adsorption on polyacrylate-coated TiO(2) engineered nanoparticles (TiO(2)-ENs) and its effect on the bioavailability as well as toxicity of Cd(2+) to a green alga Chlamydomonas reinhardtii were investigated. TiO(2)-ENs could be well dispersed in the experimental medium and their pH(pzc) is approximately 2. There was a quick adsorption of Cd(2+) on TiO(2)-ENs and a steady state was reached within 30 min. A pseudo-first order kinetics was found for the time-related cha ...[more]

PMID: 22403644

Similar Datasets

Project description:BackgroundNon-steroidal anti-inflammatory drugs (NSAIDs), such as diclofenac (DCF), form a significant group of environmental contaminants. When the toxic effects of DCF on plants are analyzed, authors often focus on photosynthesis, while mitochondrial respiration is usually overlooked. Therefore, an in vivo investigation of plant mitochondria functioning under DCF treatment is needed. In the present work, we decided to use the green alga Chlamydomonas reinhardtii as a model organism.MethodsSynchronous cultures of Chlamydomonas reinhardtii strain CC-1690 were treated with DCF at a concentration of 135.5 mg × L-1, corresponding to the toxicological value EC50/24. To assess the effects of short-term exposure to DCF on mitochondrial activity, oxygen consumption rate, mitochondrial membrane potential (MMP) and mitochondrial reactive oxygen species (mtROS) production were analyzed. To inhibit cytochrome c oxidase or alternative oxidase activity, potassium cyanide (KCN) or salicylhydroxamic acid (SHAM) were used, respectively. Moreover, the cell's structure organization was analyzed using confocal microscopy and transmission electron microscopy.ResultsThe results indicate that short-term exposure to DCF leads to an increase in oxygen consumption rate, accompanied by low MMP and reduced mtROS production by the cells in the treated populations as compared to control ones. These observations suggest an uncoupling of oxidative phosphorylation due to the disruption of mitochondrial membranes, which is consistent with the malformations in mitochondrial structures observed in electron micrographs, such as elongation, irregular forms, and degraded cristae, potentially indicating mitochondrial swelling or hyper-fission. The assumption about non-specific DCF action is further supported by comparing mitochondrial parameters in DCF-treated cells to the same parameters in cells treated with selective respiratory inhibitors: no similarities were found between the experimental variants.ConclusionsThe results obtained in this work suggest that DCF strongly affects cells that experience mild metabolic or developmental disorders, not revealed under control conditions, while more vital cells are affected only slightly, as it was already indicated in literature. In the cells suffering from DCF treatment, the drug influence on mitochondria functioning in a non-specific way, destroying the structure of mitochondrial membranes. This primary effect probably led to the mitochondrial inner membrane permeability transition and the uncoupling of oxidative phosphorylation. It can be assumed that mitochondrial dysfunction is an important factor in DCF phytotoxicity. Because studies of the effects of NSAIDs on the functioning of plant mitochondria are relatively scarce, the present work is an important contribution to the elucidation of the mechanism of NSAID toxicity toward non-target plant organisms.

Dataset Information

Cd2+ Toxicity to a green alga Chlamydomonas reinhardtii as influenced by its adsorption on TiO2 engineered nanoparticles.

Publications

Cd2+ Toxicity to a green alga Chlamydomonas reinhardtii as influenced by its adsorption on TiO2 engineered nanoparticles.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets