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Putting structure into context: fitting of atomic models into electron microscopic and electron tomographic reconstructions.

ABSTRACT: A complete understanding of complex dynamic cellular processes such as cell migration or cell adhesion requires the integration of atomic level structural information into the larger cellular context. While direct atomic-level information at the cellular level remains inaccessible, electron microscopy, electron tomography and their associated computational image processing approaches have now matured to a point where sub-cellular structures can be imaged in three dimensions at the nanometer scale. Atomic-resolution information obtained by other means can be combined with this data to obtain three-dimensional models of large macromolecular assemblies in their cellular context. This article summarizes some recent advances in this field.

SUBMITTER: Volkmann N

PROVIDER: S-EPMC3294126 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Putting structure into context: fitting of atomic models into electron microscopic and electron tomographic reconstructions.

Volkmann Niels N

Current opinion in cell biology 20111205 1

A complete understanding of complex dynamic cellular processes such as cell migration or cell adhesion requires the integration of atomic level structural information into the larger cellular context. While direct atomic-level information at the cellular level remains inaccessible, electron microscopy, electron tomography and their associated computational image processing approaches have now matured to a point where sub-cellular structures can be imaged in three dimensions at the nanometer scal ...[more]

PMID: 22152946

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